| 產(chǎn)品編號(hào) | bs-0054R |
| 英文名稱 | Rabbit Anti-GDNF Receptor alpha 2 antibody |
| 中文名稱 | 膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子受體α2抗體 |
| 別 名 | GDNF family receptor alpha-2; GDNF Receptor α2; GDNF receptor beta; GDNFR beta; GDNFR-beta; GDNFRB; GFR alpha 2; GFRA 2; GFRA2; GFR alpha-2; Glial cell line derived neurotrophic factor family receptor alpha2b; Glial cell line derived neurotrophic factor receptor beta; Neurturin receptor alpha; NRTNR alpha; NRTNR-alpha; NTNR alpha; NTNR-alpha; NTNRA; PI linked cell surface accessory protein; RET ligand 2; RETL 2; RETL2; TGF beta related neurotrophic factor receptor 2; TRN receptor GPI anchored; TRNR 2; TRNR2; GFRA2_HUMAN; GDNF family receptor alpha-2; GDNF receptor alpha-2; Short=GDNFR-alpha-2; GFR-alpha-2; Neurturin receptor alpha; TGF-beta-related neurotrophic factor receptor 2. |
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Specific References (1) | bs-0054R has been referenced in 1 publications.
[IF=3.499] Ye F et al. Nitrogen mustard prevents transport of Fra-1 into the nucleus to promote c-Fos-and FosB-dependent IL-8 induction in injured mouse epidermis. Toxicol Lett. 2019 Oct 19. pii: S0378-4274(19)30325-X. IHC-P ; Mouse.
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| 研究領(lǐng)域 | 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDNF Receptor alpha 2: 301-360/464 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene.
Members of the glial cell line-derived neurotrophic factor(GDNF) family, including GDNF and neurturin (NTN),play key roles in the control of vertebrate neuron survivaland differentiation. Physiological responses to NTN requirethe presence of a novel glycosylphosphadidylinositollinked protein NTNRa, which is a cell surface receptor forNTN. The cDNAs encoding NTNRa from human, rat,chicken, and mouse have been cloned recently. NTNRa was also termed GDNFRb, Ret ligand 2 (RETL2)or TGF-b-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRa-2 recently. GFRa-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRa-2, and the Ret PTK form a complex to transduce NTN signal andto mediate NTN function.
Function: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Isoform 1 is found in both brain and placenta. Similarity: Belongs to the GDNFR family. SWISS: O00451 Gene ID: 2675 Database links: Entrez Gene: 2675 Human Entrez Gene: 14586 Mouse Omim: 601956 Human SwissProt: O00451 Human SwissProt: O08842 Mouse 神經(jīng)生物學(xué)相關(guān)蛋白（Neurobiology） 膠質(zhì)細(xì)胞源性神經(jīng)營養(yǎng)因子(GDNF)是TGF-β家族的一個(gè)新亞族，該亞族還有新發(fā)現(xiàn)的三個(gè)成員：Neurturin(NTN)、Persephin(PSP)和Artemin(ART)，它們均不程度地對(duì)多巴胺神經(jīng)元、脊髓前角運(yùn)動(dòng)神經(jīng)元、背根神經(jīng)節(jié)、頸上神經(jīng)節(jié)以及腎等非神經(jīng)元細(xì)胞都具有生長(zhǎng)促進(jìn)作用。研究表明膠質(zhì)細(xì)胞系源性神經(jīng)營養(yǎng)因子,主要用于PD的研究。 GDNF的受體是多成分的復(fù)合物，它是由固定于質(zhì)膜外層的糖基磷脂酰肌醇(GPI)被稱為GDNFRα和酪氨酸激酶RET蛋白組成。GDNFRα為其配體結(jié)合亞單位，而RET則是GDNF的功能性受體亞單位，即GDNF特異地結(jié)合于其受體的GDNFRα上，再激活RET的酪氨酸磷酸化傳導(dǎo)其信號(hào)，影響細(xì)胞的活性，發(fā)揮其生物學(xué)效應(yīng)。目前，又克隆出了人與大鼠的GDNFRβ序列，與GDNPRα具有47％的同源性。GDNFRβ mRNA在脊髓、DRG和發(fā)育中的周圍神經(jīng)上也均有表達(dá)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Liver tissue lysates
Primary: Anti-GDNF Receptor alpha 2 (bs-0054R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- GDNF Receptor alpha 2 (bs-0054R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 52 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GDNF-Receptor-alpha-2 Polyclonal Antibody, Unconjugated(bs-0054R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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