| 產(chǎn)品編號 | bs-1296R |
| 英文名稱 | Rabbit Anti-Defensin beta 2 antibody |
| 中文名稱 | 防御素β2/Defensin β2抗體 |
| 別 名 | beta-defensin 2 precursor; beta Defensin 2; beta-defensin 2; Defb2; Q32ZI5_RAT; DFB4A_HUMAN. Defensin β2. |
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Specific References (2) | bs-1296R has been referenced in 2 publications.
[IF=3.365] Shalong Wang. et al. Bovine Lactoferrin Protects Dextran Sulfate Sodium Salt Mice Against Inflammation and Impairment of Colonic Epithelial Barrier by Regulating Gut Microbial Structure and Metabolites. Front Nutr. 2021; 8: 660598 WB,IF ; Mouse.
[IF=2.245] Renli Qi. et al. Changes of Gut Microbiota and Its Correlation With Short Chain Fatty Acids and Bioamine in Piglets at the Early Growth Stage. Front Vet Sci. 2020; 7: 617259 WB ; Pig.
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| 研究領(lǐng)域 | 微生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Rat) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 8kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat beta 2 Defensin: 21-71/71 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Jul 2008] Subcellular Location: Secreted. Tissue Specificity: Expressed in the skin and respiratory tract. Similarity: Belongs to the beta-defensin family. LAP/TAP subfamily. SWISS: Q32ZI5 Gene ID: 641631 Database links:
防御素在人體中廣泛分布的，目前已知的α－防御素有6種，研究人員從人體的腎臟、氣管、鼻粘膜與唾液中也發(fā)現(xiàn)了兩種β防御素，在許多器官的上皮細胞與非上皮組織的細胞中發(fā)現(xiàn)了β－防御素。 Defensin β2在維持肌體天然免疫功能、介導炎癥反應以及調(diào)節(jié)特異性免疫中的作用引起人們的重視，防御素的表達與病原體的入侵緊密相關(guān)。 |
| 產(chǎn)品圖片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-beta 2 Defensin Polyclonal Antibody, Unconjugated(bs-1296R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
