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Rabbit Anti-CD97  antibody (bs-2522R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-2522R
英文名稱 Rabbit Anti-CD97  antibody
中文名稱 CD97抗體
別    名 CD97 antigen subunit beta; Leukocyte antigen CD97; CD 97; CD97; CD97 antigen; CD97 molecule; CD97_HUMAN; Leukocyte antigen CD97; Seven span transmembrane protein; Seven transmembrane helix receptor; Seven transmembrane heterodimeric receptor associated with inflammation; TM7LN 1; TM7LN1.  
研究領(lǐng)域 免疫學(xué)  神經(jīng)生物學(xué)  細(xì)胞膜受體  細(xì)胞粘附分子  G蛋白偶聯(lián)受體  細(xì)胞表面分子  糖蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat,Pig,Cow,Dog)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
細(xì)胞定位 細(xì)胞膜 細(xì)胞外基質(zhì) 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD97 antigen subunit beta: 741-835/835 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011].

Function:
Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.

Subunit:
Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) interacts with chondroitin sulfate.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.
CD97 antigen subunit alpha: Secreted, extracellular space.

Tissue Specificity:
Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.

Post-translational modifications:
Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.

Similarity:
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains.
Contains 1 GPS domain.

SWISS:
P48960

Gene ID:
976

Database links:

Entrez Gene: 976 Human

Entrez Gene: 26364 Mouse

Entrez Gene: 361383 Rat

Omim: 601211 Human

SwissProt: P48960 Human

SwissProt: Q9Z0M6 Mouse

Unigene: 466039 Human

Unigene: 334648 Mouse

Unigene: 107655 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD97) Polyclonal Antibody, Unconjugated (bs-2522R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-CD97 antibody(bs-2522R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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