| 產(chǎn)品編號(hào) | bs-3666R |
| 英文名稱(chēng) | Rabbit Anti-Calmodulin 1/2/3 antibody |
| 中文名稱(chēng) | 鈣調(diào)節(jié)素/鈣調(diào)蛋白/鈣調(diào)素抗體 |
| 別 名 | CALM 1; CALM 2; CALM 3; CALM; CALM1; CALM2; CALM3; CALML2; Calmodulin 1; Calmodulin1; CAM 1; CAM 2; CAM 3; CaM; CAM I; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; DD132; PHKD; phosphorylase kinase delta; phosphorylase kinase delta subunit; Calmodulin1; Calmodulin 1; Calmodulin-1; Calmodulin2; Calmodulin 2; Calmodulin-2; Calmodulin3; Calmodulin 3; Calmodulin-3; Calmodulin; CALM1_HUMAN. |
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Specific References (4) | bs-3666R has been referenced in 4 publications.
[IF=5.561] Lei Wu. et al. Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway. FOODS. 2023 Jan;12(5):1101 WB ; Rat.
[IF=5.201] Hua Y et al. Enterohemorrhagic Escherichia coli Effector Protein EspF Interacts With Host Protein ANXA6 and Triggers Myosin Light Chain Kinase (MLCK)-Dependent Tight Junction DysregulationFront Cell Dev Biol.2020 Dec 23;8:613061. WB ; Human.
[IF=3.448] Xu B et al. Excessive?mechanical?stress?induces?chondrocyte?apoptosis?through?TRPV4?in an?anteriorcruciate?ligament-transected?rat?osteoarthritis?model. Life Sci. 2019 Jul 1;228:158-166. IHC-P&WB ; Rat.
[IF=2.824] Hai-Jun Gao. et al. Anti-echinococcal effect of verapamil involving the regulation of the calcium/calmodulin-dependent protein kinase II response in vitro and in a murine infection model. Parasite Vector. 2021 Dec;14(1):1-10 IHC ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 生長(zhǎng)因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 細(xì)胞表面分子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16-20 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calmodulin: 81-152/152 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calmodulin consists of two glycoproteins, 34 and 39 kDa, sometimes designated epithelial antigen, epithelial specific antigen, and epithelial glycoprotein. The glycoproteins are located on the cell membrane surface
and in the cytoplasm of virtually all epithelial cells with the exception of most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. Epithelial Calmodulin is found in the large majority of adenocarcinomas of most sites (50-100% in various studies; as well as neuroendocrine tumours, including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain in about 30% of the cases. Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin Ca(2+) complex are a number of protein kinases and phosphatases. Calmodulin has four functional calcium binding sites. Subunit: Interacts with MYO1C and RRAD. Interacts with MYO10 (By similarity). Interacts with CEP97, CEP110, TTN/titin and SRY. Interacts with USP6; the interaction is calcium dependent. Interacts with CDK5RAP2. Interacts with SCN5A. Interacts with RYR1 and RYR2. Subcellular Location: Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. Similarity: Belongs to the calmodulin family. Contains 4 EF-hand domains. SWISS: P0DP23 Gene ID: 801 Database links: Entrez Gene: 801 Human Entrez Gene: 12314 Mouse Entrez Gene: 12315 Mouse Omim: 114180 Human Omim: 114182 Human Omim: 114183 Human SwissProt: P62158 Human SwissProt: P62204 Mouse Unigene: 282410 Human Unigene: 468442 Human Unigene: 515487 Human Unigene: 706125 Human Unigene: 722012 Human Unigene: 285993 Mouse Unigene: 288630 Mouse Unigene: 329243 Mouse 鈣離子調(diào)節(jié)素（calmodulin,CaM）: 鈣調(diào)蛋白（CaM），是由一條多肽鏈組成的單體蛋白。可結(jié)合4個(gè)Ca2+，胞漿Ca2+濃度≥10-2mmol/L時(shí)，Ca2+與CaM結(jié)合，構(gòu)象改變而激活Ca2+ -CaM激酶。 |
| 產(chǎn)品圖片 |
Sample:
Lane1: Brain(Rat) Lysate at 30 ug
Lane2: Liver(Rat) Lysate at 30 ug
Primary: Anti-Calmodulin (bs-3666R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 16kD
Observed band size : 20kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calmodulin 1/2/3) Polyclonal Antibody, Unconjugated (bs-3666R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calmodulin 1/2/3) Polyclonal Antibody, Unconjugated (bs-3666R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calmodulin 1/2/3) Polyclonal Antibody, Unconjugated (bs-3666R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell:Human hepatocellular carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Calmodulin Polyclonal Antibody, Unconjugated(bs-3666R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calmodulin 1/2/3) Polyclonal Antibody, Unconjugated (bs-3666R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Calmodulin 1/2/3 antibody (bs-3666R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Calmodulin 1/2/3 antibody (bs-3666R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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