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Rabbit Anti-IL-2  antibody (bs-4586R)  
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產(chǎn)品編號 bs-4586R
英文名稱 Rabbit Anti-IL-2  antibody
中文名稱 白介素2抗體
別    名 Interleukin-2; Interleukin 2; Interleukin2; T-cell growth factor; T Cell Growth Factor; Aldesleukin; IL 2; IL2; Lymphokine; POIL2; T Cell Growth Factor; TCGF; Involved in regulation of T cell clonal expansion; IL2_HUMAN; IL2_MOUSE.  
Specific References  (1)     |     bs-4586R has been referenced in 1 publications.
[IF=3.776] Yang Yang. et al. The NF-κB pathway plays a vital role in rat salivary gland atrophy model. HELIYON. 2023 Mar; 9(3): e14288  IHC ;  Rat.  
研究領(lǐng)域 免疫學(xué)  糖蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 15kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse IL-2: 81-169/169 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.

Function:
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Subcellular Location:
Secreted.

DISEASE:
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.

Similarity:
Belongs to the IL-2 family.

SWISS:
P04351

Gene ID:
16183

Database links:

Entrez Gene: 3558 Human

Entrez Gene: 16183 Mouse

Entrez Gene: 116562 Rat

Omim: 147680 Human

SwissProt: P60568 Human

SwissProt: P04351 Mouse

SwissProt: P17108 Rat

Unigene: 89679 Human

Unigene: 14190 Mouse

Unigene: 9871 Rat



產(chǎn)品圖片
Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug Lane 2: Large intestine (Rat) Lysate at 40 ug Primary: Anti-IL-2 (bs-4586R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded ( human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-2 ) Polyclonal Antibody, Unconjugated (bs-4586R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded ( human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-2 ) Polyclonal Antibody, Unconjugated (bs-4586R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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