| 產(chǎn)品編號 | bs-5723R |
| 英文名稱 | Rabbit Anti-OSGIN1 antibody |
| 中文名稱 | 氧化應激誘導生長抑制因子1抗體 |
| 別 名 | BDGI; Bone marrow stromal cell derived growth inhibitor; huOKL38; OKL 38; OKL38; OSGIN 1; Ovary kidney and liver protein 38; Oxidative stress induced growth inhibitor 1; Pregnancy induced growth inhibitor; Pregnancy induced growth inhibitor OKL38; OSGI1_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 染色質(zhì)和核信號 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 61kDa |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OSGIN1: 351-450/560 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
OSGIN1 (Oxidative stress-induced growth inhibitor 1) regulates the differentiation and proliferation of normal cells through the regulation of cell death. Function: Regulates the differentiation and proliferation of normal cells through the regulation of cell death. Tissue Specificity: Ubiquitous. Highest expression in the ovary, testis, kidney, and liver. Similarity: Belongs to the OKL38 family. SWISS: Q9UJX0 Gene ID: 29948 Database links: Entrez Gene: 29948 Human Entrez Gene: 71839 Mouse Omim: 607975 Human SwissProt: Q9UJX0 Human |
| 產(chǎn)品圖片 |
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-OSGIN1 (bs-5723R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 61 kD
Observed band size: 55 kD
Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-OSGIN1 (bs-5723R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti-OSGIN1 (bs-5723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDa
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSGIN1) Polyclonal Antibody, Unconjugated (bs-5723R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
