| 產(chǎn)品編號 | bs-7507R |
| 英文名稱 | Rabbit Anti-Sprouty 2 antibody |
| 中文名稱 | 快速發(fā)育生長因子同源蛋白2抗體 |
| 別 名 | hSPRY 2; hSPRY2; MGC23039; Sprouty homolog 2 (Drosophila); Sprouty homolog 2; Sprouty2; Spry 2; Spry2; SPY2_HUMAN. |
| 研究領域 | 腫瘤 心血管 細胞生物 神經(jīng)生物學 干細胞 生長因子和激素 血管內(nèi)皮細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Sprouty 2: 65-170/315 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty 2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C terminus of the protein. Significant sequence divergence occurs at the N terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle. Function: May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Subunit: Interacts with RAF1. Subcellular Location: Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Note=Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor). Similarity: Belongs to the sprouty family. Contains 1 SPR (sprouty) domain. SWISS: O43597 Gene ID: 10253 Database links: Entrez Gene: 10253 Human Entrez Gene: 24064 Mouse Omim: 602466 Human SwissProt: O43597 Human SwissProt: Q9QXV8 Mouse Unigene: 18676 Human Unigene: 89982 Mouse Unigene: 103294 Rat Sprouty2是一類在全身組織廣泛表達的軟脂?；椎鞍?在機體的信號轉導,尤其是受體蛋白對受體酪氨酸激酶(RTK)等信號通路起著重要的抑制作用,其表達在多種腫瘤組織中發(fā)生明顯下降,并可進一步引起細胞異常增殖、轉化及新生血管形成,從而促進腫瘤的發(fā)生及發(fā)展。 |
| 產(chǎn)品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Sprouty 2 (bs-7507R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 33 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sprouty 2) Polyclonal Antibody, Unconjugated (bs-7507R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
