| 產(chǎn)品編號 | bs-6961R |
| 英文名稱 | Rabbit Anti-ASMTY antibody |
| 中文名稱 | 松果體N甲基乙酰羥色胺抗體 |
| 別 名 | acetylserotonin methyltransferase (Y chromosome); acetylserotonin N-methyltransferase; Acetylserotonin O-methyltransferase; ASMT; ASMTY; HIOM_HUMAN; HIOMT; HIOMTY; Hydroxyindole O-methyltransferase. |
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Specific References (6) | bs-6961R has been referenced in 6 publications.
[IF=5.249] Zhang, WL.. et al. Expression of arylalkylamine n-acetyltransferase (AANAT) and acetylserotonin o-methyltransferase (ASMT) in the corpus luteum of pregnant sows and synthesis of melatonin in luteal cells. 2021 Nov 24 WB,IHC ; Pig.
[IF=3.24] Herman, A. P., et al. "Central Interleukin-1β Suppresses the Nocturnal Secretion of Melatonin." Mediators of Inflammation 2016 (2016). WB ; Others.
[IF=2.71] Herman, A. P., et al. "Interleukin-1β Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study." BioMed Research International (2015). WB ; Other Species.
[IF=2.089] Huawei Wei. et al. Melatonin through blockade of Hif-1α signaling mediates the anti-fibrosis under hypoxia in canine Sertoli cells. REPROD BIOL. 2022 Dec;22:100677 WB, IHC ; Dog.
[IF=1.858] Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 Aug;: IHC ; Sheep.
[IF=0.86] Herman et al. Endotoxin-induced inflammation disturbs melatonin secretion in ewe. (2017) Asian-Australas.J.Anim.Sci. 30:1784-1795 WB ; Sheep.
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| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 內分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Dopamine transporter: 241-345/345 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Most abundant in pineal gland, less in retina and brain, not in pigment epithelium. Function: Produces melatonin (N-acetyl-5-methoxytryptamine) from N-acetylserotonin. Tissue Specificity: Expressed in the pineal gland (at protein level). In the retina, very low expression is found at the mRNA level (PubMed:7989373), and not at the protein level (PubMed:8574683). Similarity: Belongs to the methyltransferase superfamily. SWISS: P46597 Gene ID: 438 Database links: UniProtKB/Swiss-Prot: P46597.1 |
| 產(chǎn)品圖片 |
Sample: Brain (Rat) Lysate at 40 ug
Primary: Anti- ASMTY (bs-6961R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 38 kD
Observed band size: 38/45 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
