| 產(chǎn)品編號 | bs-6440R |
| 英文名稱 | Rabbit Anti-ZnT-1 antibody |
| 中文名稱 | 鋅轉(zhuǎn)運蛋白1抗體 |
| 別 名 | SLC30 A1; SLC30A 1; SLC30A1; Solute carrier family 30 (zinc transporter), member 1; Solute carrier family 30 (zinc transporter), member1; Solute carrier family 30 member 1; Solute carrier family 30, member 1; Zinc transporter 1; Zinc transporter1; ZNT 1; ZnT-1; ZNT1_HUMAN; ZRC 1; ZRC1. |
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Specific References (1) | bs-6440R has been referenced in 1 publications.
[IF=6.543] Xiao Chuanpi. et al. High Temperature-Induced Oxidative Stress Affects Systemic Zinc Homeostasis in Broilers by Regulating Zinc Transporters and Metallothionein in the Liver and Jejunum. Oxid Med Cell Longev. 2022;2022:1427335 WB ; Broiler Chicks.
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| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 轉(zhuǎn)運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZnT-1: 131-330/507 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Zinc, an essential element required for cell proliferation and differentiation, plays a role in a diverse array of cellular functions, including acting as a cofactor for numerous enzymes and transcription factors and as a neuroregulator. The zinc transporter (ZnT) family regulates the supply of zinc within cells, and its members are characterized by containing six membrane-spanning domains, a large histidine-rich intracellular loop, and a C-terminal tail. ZnT-1, a ubiquitous protein, localizes to the plasma membrane to aid in the export of zinc out of cells. Function: May be involved in zinc transport out of the cell. Subunit: Multimer. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. SWISS: Q9Y6M5 Gene ID: 7779 Database links: Entrez Gene: 7779 Human Entrez Gene: 22782 Mouse Omim: 609521 Human SwissProt: Q9Y6M5 Human SwissProt: Q60738 Mouse Unigene: 519469 Human Unigene: 9024 Mouse Unigene: 10120 Rat |
| 產(chǎn)品圖片 |
Sample:Large intestine (mouse) Lysate at 40 ug
Primary: Anti- ZnT-1(bs-6440R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55kD
Sample:Lovo cell (human) Lysate at 40 ug
Primary: Anti- ZnT-1(bs-6440R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55kD
Sample:
Lane 1: Mouse Small intestine tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Small intestine tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human U251 cell lysates
Lane 7: Human K562 cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti-ZnT-1 (bs-6440R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 50 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
