| 產(chǎn)品編號 | bs-8200R |
| 英文名稱 | Rabbit Anti-phospho-MYF5 (phospho Ser49) antibody |
| 中文名稱 | 磷酸化生肌決定因子Myf5抗體 |
| 別 名 | Myf5 (phospho S49); Myf5 (phospho Ser49); p-Myf5(phospho S49); p-Myf5(phospho Ser49); Myf-5 bHLHc2; Class C basic helix loop helix protein 2; Class C basic helix-loop-helix protein 2; Myf 5; Myf-5; Myf5; MYF5_HUMAN; Myogenic factor 5. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 發(fā)育生物學(xué) 信號轉(zhuǎn)導(dǎo) 干細胞 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human MYF5 around the phosphorylation site of Ser49: LQG(p-S)D |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Function: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Subcellular Location: Nucleus. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P13349 Gene ID: 4617 Database links: Entrez Gene: 4617 Human Entrez Gene: 17877 Mouse Omim: 159990 Human SwissProt: P13349 Human SwissProt: P24699 Mouse Unigene: 178023 Human Unigene: 4984 Mouse yf5是MyoD基因家族的一員，該家族的共同結(jié)構(gòu)特點是具有1個保守的中央蛋白基序(motif)，大小為80個氨基酸，稱為堿性螺旋-環(huán)-螺旋(bHLH)，bHLH高度保守，其中Basic結(jié)構(gòu)是HLH螺旋結(jié)構(gòu)的延伸，也是這一家族蛋白與DNA相互作用的區(qū)域，而HLH螺旋結(jié)構(gòu)則是與其他因子相互作用的位點，即調(diào)控的重要區(qū)域。 生肌決定因子(MyoD)基因家族控制著肌細胞的增殖和分化，與肌纖維的數(shù)量、大小有著密切的關(guān)系，因而對肉質(zhì)和風(fēng)味有非常重要的作用，MyoD基因家族可編碼4種不同的轉(zhuǎn)錄因子，分別為MyoD(Myf3)、Myogenin(MyoG)、Myf5、Myf6或MRF4，它們各自或協(xié)同控制著骨骼肌生成方面的關(guān)鍵調(diào)節(jié)因子。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
