| 產(chǎn)品編號 | bs-8561R |
| 英文名稱 | Rabbit Anti-Laminin 2 alpha antibody |
| 中文名稱 | 層粘蛋白α2抗體 |
| 別 名 | LAMA 2; LAMA2; LAMA2; LAMA2_HUMAN; Laminin alpha 2 (merosin congenital muscular dystrophy); Laminin alpha 2; Laminin alpha 2 chain; Laminin alpha 2 subunit; Laminin M; Laminin M chain; Laminin subunit alpha-2; Laminin-12 subunit alpha; Laminin-2 subunit alpha; Laminin-4 subunit alpha; LAMM; LAMM; Merosin heavy chain; Merosin heavy chain; Laminin-12 subunit alpha. |
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Specific References (5) | bs-8561R has been referenced in 5 publications.
[IF=5.789] Palma et al. Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma. (2018) J.Tissue.Eng. 9:2041731418810164 IHC ; horse.
[IF=4.174] Zhang, Zhao. et al. Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer. Hum Cell. 2022 Jan;:1-16 IHC ; Human.
[IF=3.99] Silva Nunes Barreto, Rodrigo, et al. "Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement." Journal of Tissue Engineering and Regenerative Medicine (2017). IHC-P ; Bovine.
[IF=2.586] R S N Barreto. et al. THE EXTRACELLULAR MATRIX PROTEIN PATTERN IN THE CANINE NEOPLASTIC MAMMARY GLAND. TISSUE CELL. 2023 Feb;:102050 IHC ; Dog.
[IF=1.638] Borghesi J et al. Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation. Reprod Domest Anim. 2019 Jul 6. IHC-P ; water buffalos.
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| 研究領(lǐng)域 | 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞粘附分子 細(xì)胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 341kDa |
| 細(xì)胞定位 | 細(xì)胞膜 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Laminin 2 alpha: 2051-2200/3122 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Jul 2008]. Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Subunit: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-2 is a subunit of laminin-2 (laminin-211 or merosin), laminin-4 (laminin-221 or S-merosin) and laminin-12 (laminin-213). Interacts with FBLN1, FBLN2 and NID2. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. Tissue Specificity: Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone. DISEASE: Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI. Similarity: Contains 17 laminin EGF-like domains. Contains 5 laminin G-like domains. Contains 2 laminin IV type A domains. Contains 1 laminin N-terminal domain. SWISS: P24043 Gene ID: 3908 Database links: Entrez Gene: 3908 Human Omim: 156225 Human SwissProt: P24043 Human Unigene: 200841 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Laminin 2 alpha) Polyclonal Antibody, Unconjugated (bs-8561R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Laminin 2 alpha) Polyclonal Antibody, Unconjugated (bs-8561R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Laminin 2 alpha) Polyclonal Antibody, Unconjugated (bs-8561R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Laminin 2 alpha) Polyclonal Antibody, Unconjugated (bs-8561R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Laminin 2 alpha Polyclonal Antibody, Unconjugated(bs-8561R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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