| 產品編號 | bs-12061R |
| 英文名稱 | Rabbit Anti-GABRA2 antibody |
| 中文名稱 | G氨基丁酸A型受體α2/GABAA Rα2抗體 |
| 別 名 | GABA A Receptor a2; GABA A Receptor alpha 2; GABA A receptor subunit alpha 2; GABA; GABA(A) receptor subunit alpha 2; GABA(A) receptor subunit alpha-2; GABR A2; GABRA 2; GABRA2; GABRA2 protein; Gamma aminobutyric acid (GABA) A receptor alpha 2; Gamma aminobutyric acid A receptor alpha 2; Gamma aminobutyric acid receptor alpha 2 subunit; Gamma aminobutyric acid receptor subunit alpha 2; Gamma-aminobutyric acid receptor subunit alpha-2; GBRA2_HUMAN. |
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Specific References (2) | bs-12061R has been referenced in 2 publications.
[IF=6.208] Yaiza M. Arenas. et al. Enhanced BDNF and TrkB Activation Enhance GABA Neurotransmission in Cerebellum in Hyperammonemia. INT J MOL SCI. 2022 Jan;23(19):11770 WB ; Rat.
[IF=5.59] Sancho-Alonso, María. et al. Hyperammonemia Enhances GABAergic Neurotransmission in Hippocampus: Underlying Mechanisms and Modulation by Extracellular cGMP. Mol Neurobiol. 2022 Mar;:1-18 WB ; Rat.
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| 研究領域 | 神經(jīng)生物學 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GABRA2: 185-280/451 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Subunit: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Subcellular Location: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily. SWISS: P47869 Gene ID: 2555 Database links: Entrez Gene: 2555 Human Entrez Gene: 14395 Mouse Omim: 137140 Human SwissProt: P47869 Human SwissProt: P26048 Mouse Unigene: 116250 Human Unigene: 5304 Mouse Unigene: 48180 Rat |
| 產品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GABRA2 (bs-12061R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Cerebral cortex(Rat) Lysate at 40 ug
Primary: Anti-GABRA2 (bs-12061R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample: Cerebrum (mouse) Lysate at 40 ug
Primary: Anti- GABRA2(bs-12061R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated(bs-12061R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- GABRA2 antibody(bs-12061R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
