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Rabbit Anti-GCNT1  antibody (bs-12334R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-12334R
英文名稱 Rabbit Anti-GCNT1  antibody
中文名稱 GCNT1葡萄糖轉(zhuǎn)移酶抗體
別    名 3-galactosyl-O-glycosyl-glycoprotein beta-1; 6-N-acetylglucosaminyltransferase; Beta-1; Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase; C2GNT; C2GNT L; C2GNT1; C2GnTL; Core 2 branching enzyme; Core 2 GNT; Core 2-branching enzyme; Core2 GlcNAc transferase; Core2-GlcNAc-transferase; GCNT 1; GCNT 1; GCNT1; GCNT1_HUMAN; glucosaminyl (N-acetyl) transferase 1 core 2; NACGT2.   
研究領(lǐng)域 腫瘤  細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  糖蛋白  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Sheep,Cow,Dog)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 50kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from Human GCNT1: 201-260/428 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 GCNT1 is expressed in a variety of cell types, including lymphocytes and mucin-producing cells. Specifically, GCNT1 expression in leukocytes regulates the synthesis of core 2 O-glycans that carry sialyl-Lewis x (sLex) oligosaccharides, which confer high affinity binding to Selectins. Downregulation of Selectin ligand expression levels has been shown to inhibit tissue infiltration. Therefore, GCNT1 represents a potential drug target for the treatment of inflammatory disorders and other pathologies involving Selectins.

Function:
Forms critical branches in O-glycans.

Subcellular Location:
Golgi apparatus membrane.

Tissue Specificity:
Highly expressed in activated T-lymphocytes and myeloid cells.

Similarity:
Belongs to the glycosyltransferase 14 family.

SWISS:
Q02742

Gene ID:
2650

Database links:

Entrez Gene: 2650 Human

Entrez Gene: 14537 Mouse

Omim: 600391 Human

SwissProt: Q02742 Human

SwissProt: Q09324 Mouse

Unigene: 521568 Human

Unigene: 244825 Mouse



產(chǎn)品圖片
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- GCNT1 (bs-12334R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Sample: U87MG (Human) Cell Lysate at 30 ug Primary: Anti- GCNT1 (bs-12334R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 52 kD
Sample: Lane 1: Rat Lymph node tissue lysates Lane 2: Human SW480 cell lysates Lane 3: Human TT cell lysates Lane 4: Human U87MG cell lysates Lane 5: Human MCF-7 cell lysates Primary: Anti-GCNT1 (bs-12334R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCNT1) Polyclonal Antibody, Unconjugated (bs-12334R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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