| 產(chǎn)品編號 | bs-12343R |
| 英文名稱 | Rabbit Anti-TRIB1 antibody |
| 中文名稱 | G蛋白偶聯(lián)誘導(dǎo)蛋白2受體抗體 |
| 別 名 | Tribbles homolog 1; TRB-1; G-protein-coupled receptor-induced gene 2 protein; GIG-2; SKIP1; TRIB1_HUMAN; C8FW; GIG2; TRB1; Trib1; Tribbles homolog 1. |
| 研究領(lǐng)域 | 發(fā)育生物學(xué) 信號轉(zhuǎn)導(dǎo) 干細(xì)胞 細(xì)胞凋亡 激酶和磷酸酶 細(xì)胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Monkey,Horse) |
| 產(chǎn)品應(yīng)用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human TRIB1/GIG2: 151-250/372 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TRB-1 is a 372 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase superfamily. Expressed ubiquitously with highest expression in bone marrow, thyroid gland, skeletal muscle and pancreas, TRB-1 interacts with MAPK kinases and is thought to regulate the activation of MAP kinses, possibly controlling MAP kinase cascades. The gene encoding TRB-1 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. Function: Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity. Subcellular Location: cytoplasm. Tissue Specificity: Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily. Contains 1 protein kinase domain. SWISS: Q96RU8 Gene ID: 10221 Database links: Entrez Gene: 10221 Human Entrez Gene: 211770 Mouse Omim: 609461 Human SwissProt: Q96RU8 Human SwissProt: Q8K4K4 Mouse Unigene: 444947 Human Unigene: 40298 Mouse Unigene: 204391 Rat Trib1在脂肪組織維持和脂質(zhì)代謝紊亂上發(fā)揮關(guān)鍵作用; ????巨噬細(xì)胞至少有兩個(gè)亞類，即M1型和M2型。M1型巨噬細(xì)胞參與促炎反應(yīng)，且在宿主防御細(xì)菌和病毒感染中發(fā)揮核心作用。M2巨噬細(xì)胞與抗炎反應(yīng)、寄生蟲感染、組織重構(gòu)、纖維化以及腫瘤疾病發(fā)展相關(guān)。 Trib1是個(gè)銜接蛋白，與泛素連接酶COP1相互作用參與蛋白降解。人類全基因組關(guān)聯(lián)研究表明TRIB1參與脂質(zhì)代謝。 ????日前，大阪大學(xué)WPI免疫前沿研究中心等處的研究人員，發(fā)現(xiàn)Trib1對F4/80 + MR +組織內(nèi)在巨噬細(xì)胞的分化至關(guān)重要，且這些巨噬細(xì)胞和M2巨噬細(xì)胞、酸性粒細(xì)胞具有共同的特性(稱之為M2類巨噬細(xì)胞)。但是，Trib1在M1髓系細(xì)胞就不會發(fā)揮這種作用。相關(guān)研究成果刊登在近期出版的《自然》（Nature）雜志上。 ????Trib1缺陷導(dǎo)致各種器官中M2類巨噬細(xì)胞急劇減少，如骨髓、脾臟、肺以及脂肪組織。在Trib1缺失的骨髓細(xì)胞中，C / EBP α的異常表達(dá)要為巨噬細(xì)胞分化缺陷負(fù)責(zé)。沒有想到的是，造血細(xì)胞缺乏 Trib1的小鼠，減少了脂肪組織塊，并伴隨著脂肪分解增加，即使是在正常飲食條件下。 ????M2類巨噬細(xì)胞的補(bǔ)充可緩解這種病理學(xué)現(xiàn)象。這表明，這類巨噬細(xì)胞的缺乏是引發(fā)脂肪分解的原因。為了應(yīng)對高脂肪飲食，造血細(xì)胞缺乏 Trib1的小鼠發(fā)育中會呈現(xiàn)高甘油三酯血癥、胰島素抗性，同時(shí)增加促炎細(xì)胞基因誘導(dǎo)。 ????這些結(jié)果表明Trib1在脂肪組織維持和脂質(zhì)代謝紊亂上發(fā)揮關(guān)鍵作用，而其作用是通過控制組織內(nèi)在M2類巨噬細(xì)胞的分化實(shí)現(xiàn)的。 |
