| 產品編號 | bs-14278R |
| 英文名稱 | Rabbit Anti-DEPDC1B/BRCC3 antibody |
| 中文名稱 | 乳腺癌細胞3抗體 |
| 別 名 | BRCC3; Breast cancer cell 3; DEP domain containing 1B; DEP domain containing protein 1B; FLJ11252; DEP1B_HUMAN; HBV X transactivated gene 8 protein; HBV X-transactivated gene 8 protein; HBV XAg-transactivated protein 8; HBxAg transactivated protein 1; OTTHUMP00000122605; OTTHUMP00000221973; XTP1; XTP8. |
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Specific References (3) | bs-14278R has been referenced in 3 publications.
[IF=3.784] Z-D Liu. et al. Aberrantly high DEPDC1B expression leads to poor prognosis in patients with lower-grade gliomas. EUR REV MED PHARMACO. 2022 Nov;26(21):7813-7826 IHC ; Human.
[IF=1.664] Ma et al. High levels of glioma tumor suppressor candidate region gene 1 predicts a poor prognosis for prostate cancer. (2018) Oncol.Lett. 16:6749-6755 IHC ; Human.
[IF=1.39] Bai, Shoumin, et al. "High levels of DEPDC1B predict shorter biochemical recurrence?free survival of patients with prostate cancer." Oncology Letters. WB, IHC-P ; Human.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Cow) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DEPDC1B/BRCC3: 451-529/529 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GTPase activator activity. biological process: intracellular signaling cascade Subcellular Location: intracellular Similarity: Contains 1 DEP domain. Contains 1 Rho-GAP domain. SWISS: Q8WUY9 Gene ID: 55789 Database links: Entrez Gene: 55789 Human SwissProt: Q8WUY9 Human |
| 產品圖片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti-DEPDC1B/BRCC3 (bs-14278R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 68 kDa
Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BRCC3 Polyclonal Antibody, Unconjugated(bs-14278R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
