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Rabbit Anti-ERp57  antibody (bs-14629R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號(hào) bs-14629R
英文名稱 Rabbit Anti-ERp57  antibody
中文名稱 內(nèi)質(zhì)網(wǎng)蛋白57抗體
別    名 ERp57/ERp60; 58 kDa glucose regulated protein; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER 60; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER p57; ER protein 57; ER protein 60; ERp 57; ERp57; ERp60; ERp61; Glucose Regulated Protein 58 Kd; GRP 57; GRP 58; GRP57; GRP58; HsT17083; P58; PDIA 3; PDIA3; PDIA3_HUMAN; Phospholipase C alpha; PI PLC; Protein disulfide isomerase A3; Protein disulfide isomerase family A member 3; Protein disulfide-isomerase A3.  
Specific References  (1)     |     bs-14629R has been referenced in 1 publications.
[IF=3.548] Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350.  WB ;  Mouse.  
研究領(lǐng)域 細(xì)胞生物  轉(zhuǎn)運(yùn)蛋白  糖蛋白  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Chicken)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 54kDa
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ERp57: 351-450/505 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

Subcellular Location:
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity:
Detected in the flagellum and head region of spermatozoa (at protein level).

Similarity:
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.

SWISS:
P30101

Gene ID:
2923

Database links:

Entrez Gene: 2923 Human

Entrez Gene: 14827 Mouse

Entrez Gene: 100328685 Rabbit

Entrez Gene: 29468 Rat

Omim: 602046 Human

SwissProt: P30101 Human

SwissProt: P27773 Mouse

SwissProt: P11598 Rat

Unigene: 591095 Human

Unigene: 263177 Mouse

Unigene: 11527 Rat



產(chǎn)品圖片
Sample: Lane 1: Mouse Skin tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Placenta tissue lysates Lane 4: Rat Testis tissue lysates Lane 5: Human A375 cell lysates Lane 6: Human HepG2 cell lysates Lane 7: Human HeLa cell lysates Primary: Anti-ERp57 (bs-14629R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 55 kDa
Sample: embryo (Mouse) Lysate at 40 ug Primary: Anti-ERp57 (bs-14629R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ERp57) Polyclonal Antibody, Unconjugated (bs-14629R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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