| 產(chǎn)品編號 | bs-16077R |
| 英文名稱 | Rabbit Anti-FGD1 antibody |
| 中文名稱 | FGD1蛋白抗體 |
| 別 名 | AAS; Faciogenital dysplasia 1 protein; FGD1; FGD1_HUMAN; FGDY; FYVE RhoGEF and PH domain containing protein 1; FYVE, RhoGEF and PH domain-containing protein 1; Rho/Rac GEF; Rho/Rac guanine nucleotide exchange factor FGD1; ZFYVE3; Zinc finger FYVE domain containing protein 3; Zinc finger FYVE domain-containing protein 3. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 發(fā)育生物學(xué) 信號轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 107kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FGD1: 601-700/961 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Activates CDC42, a member of the Ras-like family of Rho-and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Function: Activates CDC42, a member of the Ras-like family of Rho-and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Subcellular Location: Cytoplasm. Cell projection > lamellipodium. Cell projection > ruffle. Cytoplasm > cytoskeleton. Associated with membrane ruffles and lamellipodia. Tissue Specificity: Expressed in fetal heart, brain, lung, kidney and placenta. Less expressed in liver; adult heart, brain, lung, pancreas and skeletal muscle. DISEASE: Defects in FGD1 are the cause of Aarskog-Scott syndrome (AAS) [MIM:305400]. This faciogenital dysplasia is a rare multisystemic disorder characterized by disproportionately short stature, and by facial, skeletal, and urogenital anomalies. Note=Defects in FGD1 are found in a pateint with non-syndromal X-linked mental retardation. Similarity: Contains 1 DH (DBL-homology) domain. Contains 1 FYVE-type zinc finger. Contains 2 PH domains. SWISS: P98174 Gene ID: 2245 Database links: Entrez Gene: 2245 Human Omim: 305400 Human SwissProt: P98174 Human Unigene: 631767 Human Unigene: 709201 Human |
| 產(chǎn)品圖片 |
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-FGD1 (bs-16077R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 107 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGD1) Polyclonal Antibody, Unconjugated (bs-16077R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGD1) Polyclonal Antibody, Unconjugated (bs-16077R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
