| 產(chǎn)品編號 | bs-18922R |
| 英文名稱 | Rabbit Anti-TMEM175 antibody |
| 中文名稱 | 跨膜蛋白175抗體 |
| 別 名 | MGC4618; Hypothetical protein DKFZp547K246; hypothetical protein LOC84286; Hypothetical protein MGC4618; TM175_HUMAN; tmem175; Transmembrane protein 175. |
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Specific References (1) | bs-18922R has been referenced in 1 publications.
[IF=5.682] Palomba, Nicole Piera. et al. Common and Rare Variants in TMEM175 Gene Concur to the Pathogenesis of Parkinson’s Disease in Italian Patients. MOL NEUROBIOL. 2023 Jan;:1-24 IHC ; Mouse.
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| 研究領域 | 細胞生物 免疫學 跨膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TM175: 231-330/504 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TMEM175 (Transmembrane Protein 175) is a Protein Coding gene. Function: Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel. Subunit: Homodimer. Subcellular Location: Membrane. Tissue Specificity: Widely expressed. Similarity: Belongs to the TMEM175 family. SWISS: Q9BSA9 Gene ID: 84286 Database links: Entrez Gene: 84286 Human Entrez Gene: 72392 Mouse SwissProt: Q9BSA9 Human SwissProt: Q9CXY1 Mouse Unigene: 478936 Human Unigene: 258324 Mouse Unigene: 159765 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Liver (Mouse) Lysate at 40 ug
Lane 4: Testis (Mouse) Lysate at 40 ug
Lane 5: Lung (Mouse) Lysate at 40 ug
Lane 6: Spleen (Mouse) Lysate at 40 ug
Lane 7: Cerebral cortex (Rat) Lysate at 40 ug
Lane 8: Liver (Rat) Lysate at 40 ug
Lane 9: Testis (Rat) Lysate at 40 ug
Primary: Anti-TMEM175 (bs-18922R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56/43 kD
Observed band size: 45 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
