| 產(chǎn)品編號 | bs-19752R |
| 英文名稱 | Rabbit Anti-SH3BP5 antibody |
| 中文名稱 | SH3結構域結合蛋白5抗體 |
| 別 名 | 3BP5_HUMAN; OTTHUMP00000208289; OTTHUMP00000208291; OTTHUMP00000208294; SAB; SH3 binding protein; SH3 domain binding protein 5 (BTK associated); SH3 domain binding protein 5; SH3 domain binding protein that preferentially associates with BTK; SH3 domain-binding protein 5; SH3 domain-binding protein that preferentially associates with BTK; SH3 domainbinding protein 5; SH3BP 5; SH3BP-5; Sh3bp5. |
| 研究領域 | 細胞生物 信號轉(zhuǎn)導 細胞凋亡 激酶和磷酸酶 淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Zebrafish,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SH3BP5: 1-100/455 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death. Subcellular Location: Mitochondrion. Tissue Specificity: Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate. Similarity: Belongs to the SH3BP5 family. SWISS: O60239 Gene ID: 9467 Database links: Entrez Gene: 9467 Human Entrez Gene: 80851 Human Entrez Gene: 24056 Mouse Entrez Gene: 79566 Mouse Omim: 605612 Human SwissProt: O60239 Human SwissProt: Q7L8J4 Human SwissProt: Q99LH9 Mouse SwissProt: Q9Z131 Mouse Unigene: 11005 Cow Unigene: 257761 Human Unigene: 726063 Human Unigene: 290534 Mouse Unigene: 383198 Mouse Unigene: 163321 Rat |
| 產(chǎn)品圖片 |
Sample:
Ovary (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Jurkat (Human) Cell Lysate at 30 ug
Primary: Anti-SH3BP5 (bs-19752R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
