產(chǎn)品編號 | bsm-33119M |
英文名稱 | Mouse Anti-HSV-Tag antibody |
中文名稱 | HSV tag標簽單克隆抗體 |
別 名 | HSV epitope tag; QPELAPEDPED tag; Envelope glycoprotein D (Human herpesvirus 1); Envelope glycoprotein D; US6 (Human herpesvirus 1); gD. |
Specific References (1) | bsm-33119M has been referenced in 1 publications.
[IF=10.435] Gu, Kui. et al. Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken. J Nanobiotechnol. 2022 Dec;20(1):1-18 IE,LISA ; Nanobodies.
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產(chǎn)品類型 | 標簽抗體 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 7B2 |
交叉反應(yīng) | Recombinant protein |
產(chǎn)品應(yīng)用 | WB=1:1000-5000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide HSV-Tag |
亞 型 | IgG1 |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The HSV (herpes simplex virus) epitope tag is frequently engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized HSV peptide epitope represents the amino acid sequence QPELAPEDPED. HSV Tag antibody can recognize C-terminal, internal, and N-terminal HSV-tagged proteins. SWISS: N/A Gene ID: N/A Database links:
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產(chǎn)品圖片 |
Sample: Lane1: HSV-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: HSV-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-HSV tag (bsm-33119M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD
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1、抗體溶解方法 | |
2、抗體修復(fù)方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |