產(chǎn)品編號 | bsm-33080M |
英文名稱 | Mouse Anti-ABCB5 antibody |
中文名稱 | ATP結(jié)合蛋白家族5單克隆抗體 |
別 名 | ATP-binding cassette sub-family B member 5; ABCB 5; ABCB5alpha; ABCB5beta; ATP binding cassette sub family B (MDR/TAP) member 5; ATP binding cassette sub family B member 5; EST422562; P glycoprotein ABCB5; ABCB5_HUMAN. |
研究領域 | 腫瘤 信號轉(zhuǎn)導 通道蛋白 糖蛋白 |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 11B4 |
交叉反應 | Human |
產(chǎn)品應用 | WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 89kDa |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ABCB5 |
亞 型 | IgG |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008] Function: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues. Similarity: Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Contains 1 ABC transmembrane type-1 domain. Contains 2 ABC transporter domains. SWISS: Q2M3G0 Gene ID: 340273 Database links: Entrez Gene: 340273 Human Omim: 611785 Human SwissProt: Q2M3G0 Human Unigene: 404102 Human |
產(chǎn)品圖片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human HL-60 cell lysates
Primary: Anti-ABCB5 (bsm-33080M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 89 kDa
Observed band size: 85 kDa
Paraformaldehyde-fixed, paraffin embedded (human kidney carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (bsm-33080M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (bsm-33080M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ABCB5) Monoclonal Antibody, Unconjugated (bsm-33080M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |