| 產品編號 | bs-34202R |
| 英文名稱 | Rabbit Anti-LDHA antibody |
| 中文名稱 | 乳酸脫氫酶抗體 |
| 別 名 | Lactate Dehydrogenase; Lactate Dehydrogenase Isoenzyme V; Lactate Dehydrogenase isozyme H4; L lactate dehydrogenase A chain; Lactate dehydrogenase A; Lactate dehydrogenase A chain; LDH A; LDH heart subunit; LDH M; LDHM; LDH muscle subunit; LDH1; LDHA; PIG 19; PIG19; Proliferation inducing gene 19 protein; TRG 5; TRG5; LDHA_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 激酶和磷酸酶 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:10-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LDHA |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]. Subunit: Homotetramer. Subcellular Location: Cytoplasm. Post-translational modifications: ISGylated. DISEASE: Glycogen storage disease 11 (GSD11) [MIM:612933]: A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the LDH/MDH superfamily. LDH family. SWISS: P00338 Gene ID: 3939 Database links: Entrez Gene: 3939 Human Entrez Gene: 16828 Mouse Omim: 150000 Human SwissProt: P00338 Human SwissProt: P06151 Mouse Unigene: 2795 Human Unigene: 29324 Mouse Unigene: 107896 Rat |
| 產品圖片 |
Sample:
Lane 1: Muscle (Mouse) Tissue Lysate at 40 ug
Lane 2: Liver (Mouse) TissueLysate at 40 ug
Lane 3: Kidney (Mouse) Tissue Lysate at 40 ug
Lane 4: Small intestine (Mouse) Tissue Lysate at 40 ug
Lane 5: Muscle (Rat) Tissue Lysate at 40 ug
Lane 6: Liver (Rat) Tissue Lysate at 40 ug
Lane 7: Hela (Human) Cell Lysate at 30 ug
Lane 8: 293T (Human) Cell Lysate at 30 ug
Lane 9: MCF-7 (Human) Cell Lysate at 30 ug
Lane 10: Molt-4 (Human) Cell Lysate at 30 ug
Primary:
Anti-LDHA (bs-34202R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
