產(chǎn)品編號(hào) | bs-60035R |
英文名稱 | Rabbit Anti-Acetyl-Histone H4 (Lys12) antibody |
中文名稱 | |
產(chǎn)品類型 | 乙酰化抗體 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human (predicted: Mouse,Rat) |
產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
細(xì)胞定位 | 細(xì)胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
亞 型 | IgG |
純化方法 | Antigen affinity purification |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 | 組蛋白翻譯后修飾(PTMs)是表觀遺傳學(xué)調(diào)控染色質(zhì)結(jié)構(gòu)的關(guān)鍵機(jī)制,被稱為“組蛋白密碼”。組蛋白上的PTMs包括乙?;?、甲基化、磷酸化和新的?;苯佑绊懭旧|(zhì)對(duì)轉(zhuǎn)錄因子的可及性和其他表觀遺傳調(diào)控,改變基因組的穩(wěn)定性,基因轉(zhuǎn)錄等。組蛋白乙?;饕l(fā)生在核心組蛋白氨基末端的多個(gè)賴氨酸殘基上,以響應(yīng)各種刺激,在基因表達(dá)調(diào)控、DNA損傷修復(fù)、染色質(zhì)動(dòng)力學(xué)等方面發(fā)揮重要作用。大多數(shù)情況下,組蛋白H2A主要在Lys5、9、15和36位處發(fā)生乙酰化修飾。 H2B主要在Lys5、12、15、16和20位處發(fā)生乙?;揎?。組蛋白H3主要在Lys4、9、14、18、23、27、56和79位處發(fā)生乙?;揎棥=M蛋白H4主要在Lys5、8、12、16和20位處發(fā)生乙?;揎?。組蛋白乙酰轉(zhuǎn)移酶(HATs)和組蛋白去乙?;福℉DACs)是主要的調(diào)節(jié)因子。 |
產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bs-60035R
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