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Mouse Anti-IL-6  antibody (bsm-43079M)  
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產(chǎn)品編號 bsm-43079M
英文名稱 Mouse Anti-IL-6  antibody
中文名稱 白介素6單克隆抗體
別    名 IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; Interleukin-6; IL6_HUMAN; Interleukin6.  
研究領(lǐng)域 腫瘤  細胞生物  免疫學(xué)  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  生長因子和激素  激酶和磷酸酶  糖蛋白  
抗體來源 Mouse
克隆類型 Monoclonal
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 23kDa
細胞定位 細胞漿 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human IL-6 protein: 30-212/212 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].

SWISS:
P05231

Gene ID:
3569

Database links:

Entrez Gene: 3569 Human

Entrez Gene: 16193 Mouse

Entrez Gene: 24498 Rat

Omim: 147620 Human

SwissProt: P05231 Human

SwissProt: P08505 Mouse

SwissProt: P20607 Rat

Unigene: 654458 Human

Unigene: 1019 Mouse

Unigene: 9873 Rat



產(chǎn)品圖片
THP-1 (H) cells were treated with or without TPA (80 nM) and LPS (100 ng/ml,6h) and Brefeldin A (300 ng/ml,3h) , 25 μg total protein per lane of cell lysates (see on figure) probed with IL-6 monoclonal antibody, unconjugated (bsm-43079M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Sample: Lane 1: Recombinant human IL-6 protein(bs-43079P) Primary: Anti-IL-6 (bsm-43079M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
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