| 產品編號 | bs-0648R-PE |
| 英文名稱1 | Rabbit Anti-CD8/PE Conjugated antibody |
| 中文名稱 | PE標記的CD8抗體 |
| 別 名 | CD 8a; CD8 alpha chain; CD8; CD8 antigen alpha chain; CD8 antigen alpha polypeptide; CD8 antigen alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; Leu 2; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; OTTHUMP00000160760; OTTHUMP00000160764; OTTHUMP00000203528; OTTHUMP00000203721; p32; T cell antigen Leu2; T cell co receptor; T cell coreceptor; T cell surface glycoprotein CD8 alpha chain; T lymphocyte differentiation antigen T8/Leu 2; T lymphocyte differentiation antigen T8/Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen; Cd8a; Lyt-2; Lyt2. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 免疫學 干細胞 細胞表面分子 細胞類型標志物 淋巴細胞 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, |
| 產品應用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 27kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD8 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles. |
| 產品介紹 |
background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]. Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. Database links: Entrez Gene: 925 Human Entrez Gene: 926 Human Entrez Gene: 12525 Mouse Entrez Gene: 12526 Mouse Omim: 186910 Human SwissProt: P01732 Human SwissProt: P10966 Human SwissProt: P01731 Mouse SwissProt: P10300 Mouse Unigene: 405667 Human Unigene: 85258 Human Unigene: 1858 Mouse Unigene: 333148 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品圖片 |
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CD8 antibody (bs-0648R-PE)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-CD8 antibody (bs-0648R-PE)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
