產(chǎn)品編號 | bs-0245R-Bio |
英文名稱1 | Rabbit Anti-ELP1/Biotin Conjugated antibody |
中文名稱 | 生物素標記的擬南芥IKI3抗體 |
別 名 | IKI3 family protein; Elongator complex protein 1; AtELP1; Elongator component 1; Protein ABA-OVERLY SENSITIVE 1; Protein ELONGATA 2; ELP1_ARATH. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
產(chǎn)品類型 | 植物抗體 |
研究領域 | 植物 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Arabidopsis Thaliana, ) |
產(chǎn)品應用 | IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 147kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis thaliana IKI3 family protein C-terminus |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: IKI3 family is the Members of this family are components of the elongator multi-subunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation. Function: Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4 (By similarity). Promotes organs development by modulating cell division rate. Required for auxin distribution or signaling. Prevents abscisic acid (ABA) signaling leading to stomatal closure and seedling growth inhibition. Involved in oxidative stress signaling. Prevents anthocyanins accumulation. Subunit: Component of the RNA polymerase II elongator complex (Elongator) made of at least six subunits, ELP1/ELO2, ELP2, ELP3/ELO3, ELP4/ELO1, ELP5, and ELP6. Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Tissue Specificity: Expressed in meristematic tissues of in roots, stems, leaves, seedlings, cotyledons, guard cells, floral buds, flowers, siliques, and shoot apices. Similarity: Belongs to the ELP1/IKA1 family. Contains 7 WD repeats. Database links: UniProtKB/Swiss-Prot: Q9FNA4.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 擬南芥是一種十字花科植物,廣泛用于植物遺傳學、發(fā)育生物學和分子生物學的研究,已成為一種典型的模式植物。 由于這種植物的全部基因組測序已經(jīng)完成,因此可以預測,擬南芥在植物學所有領域的研究中將發(fā)揮更大的作用。雖然這種植物在許多方面"簡單",但它的大多數(shù)基因與其他"復雜"的植物基因具有很高的同源性。 該植物具有以下特點: (1)形態(tài)個體小,高度只有3Ocm左右; (2)生長周期快,從播種到收獲種子一般只需6周左右; (3)種子多,每株每代可產(chǎn)生數(shù)千粒種子; (4)形態(tài)特征簡單; (5)基因組小,只有5對染色體。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |